5Y0D

Crystal Structure of the human nucleosome containing the H2B E76K mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-15
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	98.992, 107.316, 167.719
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.582 - 1.990
R-factor	0.2127
Rwork	0.211
R-free	0.25210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cv5
RMSD bond length	0.011
RMSD bond angle	1.290
Data scaling software	HKL-2000
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.050
High resolution limit [Å]	1.980	4.260	1.980
Rmerge	0.072	0.052	0.488
Rmeas	0.077	0.054	0.562
Rpim	0.024	0.015	0.270
Total number of observations	842492
Number of reflections	118985	12772	11092
<I/σ(I)>	14.7
Completeness [%]	96.9	99.8	91.5
Redundancy	7.1	12.1	3.5
CC(1/2)		0.999	0.698

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	potassium cacodylate, potassium chloride, manganese chloride