5Y09
Crystal structure of Deg9 at 295 K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | I 4 |
Unit cell lengths | 132.372, 132.372, 154.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.888 - 2.449 |
R-factor | 0.2084 |
Rwork | 0.206 |
R-free | 0.25330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5il9 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.894 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.449 |
Number of reflections | 48768 |
<I/σ(I)> | 26.2 |
Completeness [%] | 99.7 |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.2M ammonium acetate, 0.1M HEPES pH 7.5, 45% v/v MPD |