5Y01
Acid-tolerant monomeric GFP, Gamillus, non-fluorescence (OFF) state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-16 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.900 |
Spacegroup name | I 21 3 |
Unit cell lengths | 161.797, 161.797, 161.797 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 80.900 - 2.650 |
R-factor | 0.17366 |
Rwork | 0.173 |
R-free | 0.19582 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.024 |
RMSD bond angle | 2.568 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 114.000 | 2.700 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.080 | 0.763 |
Number of reflections | 20148 | |
<I/σ(I)> | 22.6 | |
Completeness [%] | 98.9 | |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | Ammonium phosphate, sodium citrate, sodium chloride, pH3.0 |