5XZO
Crystal structure of GH10 xylanase XYL10C from Bispora. sp MEY-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL15A1 |
Synchrotron site | NSRRC |
Beamline | BL15A1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00919 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 135.457, 83.262, 65.279 |
Unit cell angles | 90.00, 94.49, 90.00 |
Refinement procedure
Resolution | 24.852 - 1.500 |
R-factor | 0.1888 |
Rwork | 0.188 |
R-free | 0.21350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4f8x |
RMSD bond length | 0.006 |
RMSD bond angle | 0.917 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11_2567)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 25.000 |
High resolution limit [Å] | 1.500 |
Number of reflections | 113828 |
<I/σ(I)> | 5.8 |
Completeness [%] | 99.9 |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298.15 | 19% PEG 3350, 0.4 M magnesium chloride, 0.1 M BIS-TRIS HYDROCHLORIDE pH 6.5 |