5XZO
Crystal structure of GH10 xylanase XYL10C from Bispora. sp MEY-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00919 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.457, 83.262, 65.279 |
| Unit cell angles | 90.00, 94.49, 90.00 |
Refinement procedure
| Resolution | 24.852 - 1.500 |
| R-factor | 0.1888 |
| Rwork | 0.188 |
| R-free | 0.21350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4f8x |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.917 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11_2567)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 1.500 |
| Number of reflections | 113828 |
| <I/σ(I)> | 5.8 |
| Completeness [%] | 99.9 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298.15 | 19% PEG 3350, 0.4 M magnesium chloride, 0.1 M BIS-TRIS HYDROCHLORIDE pH 6.5 |
