5XZG
Mouse cGAS bound to the inhibitor RU521
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2016-12-08 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 85.562, 98.430, 130.372 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.215 - 1.828 |
R-factor | 0.1958 |
Rwork | 0.194 |
R-free | 0.23170 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.153 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.215 | |
High resolution limit [Å] | 1.828 | 1.828 |
Number of reflections | 45239 | |
<I/σ(I)> | 17.7 | |
Completeness [%] | 92.3 | |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES, pH 6.9, 22.5% PEG400, 0.08 M magnesium chloride |