5XZ1

Crystal structure of the Homo Sapiens cytoplasmic ribosomal decoding site in complex with G418

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-23
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.1
Spacegroup name	P 1 21 1
Unit cell lengths	47.399, 32.528, 96.484
Unit cell angles	90.00, 95.58, 90.00

Refinement procedure

Resolution	48.013 - 3.008
Rwork	0.193
R-free	0.19900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.348
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX (1.8.3_1479)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.013	48.013	3.090
High resolution limit [Å]	3.010	13.450	3.010
Rmerge	0.070	0.031	0.356
Rmeas	0.084	0.038	0.428
Total number of observations	19672
Number of reflections	5988	81	412
<I/σ(I)>	12.46	25.84	2.53
Completeness [%]	98.5	95.3	95.8
Redundancy	3.285	2.247	3.005
CC(1/2)	0.998	0.999	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	2-methyl-2,4-pentandiol, Sodium cacodylate, Spermine tetrahydrochloride