5XYW
Crystal structure of drosophila simulans Rhino chromoshadow domain in complex with N-terminal domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.978 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 46.893, 46.893, 250.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.942 - 2.705 |
R-factor | 0.2494 |
Rwork | 0.243 |
R-free | 0.31110 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.339 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 14516 | 14516 |
<I/σ(I)> | 39 | 3.6 |
Completeness [%] | 99.8 | 98.5 |
Redundancy | 21.4 | 12.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 2.5 M NaCl, 0.1 M Tris (pH7.0), 0.2 M MgCl2 |