5XYN

The crystal structure of Csm2-Psy3-Shu1-Shu2 complex from budding yeast

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-09
Detector	PSI PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 43 21 2
Unit cell lengths	174.240, 174.240, 102.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.330 - 3.300
R-factor	0.21929
Rwork	0.217
R-free	0.25426
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.468
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.420
High resolution limit [Å]	3.300	7.100	3.300
Rmerge	0.089	0.027	0.692
Rmeas	0.097	0.030	0.754
Rpim	0.037	0.012	0.289
Number of reflections	24375		2396
<I/σ(I)>	5.9
Completeness [%]	99.9	99.3	100
Redundancy	6.5	6.2	6.3
CC(1/2)		0.998	0.791

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1M Bis-Tris, pH 6.5, 0.2M potassium sodium tartrate, 10% PEG 10000