5XYN
The crystal structure of Csm2-Psy3-Shu1-Shu2 complex from budding yeast
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-09 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9785 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 174.240, 174.240, 102.870 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.330 - 3.300 |
R-factor | 0.21929 |
Rwork | 0.217 |
R-free | 0.25426 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.468 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.420 |
High resolution limit [Å] | 3.300 | 7.100 | 3.300 |
Rmerge | 0.089 | 0.027 | 0.692 |
Rmeas | 0.097 | 0.030 | 0.754 |
Rpim | 0.037 | 0.012 | 0.289 |
Number of reflections | 24375 | 2396 | |
<I/σ(I)> | 5.9 | ||
Completeness [%] | 99.9 | 99.3 | 100 |
Redundancy | 6.5 | 6.2 | 6.3 |
CC(1/2) | 0.998 | 0.791 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.1M Bis-Tris, pH 6.5, 0.2M potassium sodium tartrate, 10% PEG 10000 |