5XYK
Structure of Transferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 50.059, 50.059, 201.242 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 67.080 - 2.570 |
R-factor | 0.26242 |
Rwork | 0.261 |
R-free | 0.28586 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5h5y |
RMSD bond length | 0.014 |
RMSD bond angle | 1.653 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 67.080 |
High resolution limit [Å] | 2.570 |
Number of reflections | 9052 |
<I/σ(I)> | 3.4 |
Completeness [%] | 94.9 |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION, RECRYSTALLIZATION | 5.5 | 290 | 0.2M ammonium acetate 0.1M Bis-Tris pH5.5 25% PEG2250 |