5XYG
Structure of 6-aminohexanoate-oligomer hydrolase from Arthrobacter sp. KI72.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.844, 144.903, 129.045 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.600 |
| R-factor | 0.16238 |
| Rwork | 0.161 |
| R-free | 0.19239 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3axg |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.514 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.055 | 0.451 |
| Number of reflections | 86374 | 5510 |
| <I/σ(I)> | 30.4 | 3.45 |
| Completeness [%] | 99.5 | 96.3 |
| Redundancy | 6.9 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.2-2.2M ammonium sulphate, 0.2M NaCl, 0.1M HEPES buffer pH 7.5-8.5 |
