5XXS
Crystal structure of native ribT from Bacillus subtilis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-27 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.95372 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.580, 57.444, 74.528 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.950 - 2.090 |
R-factor | 0.20662 |
Rwork | 0.200 |
R-free | 0.26691 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xxr |
RMSD bond length | 0.008 |
RMSD bond angle | 1.338 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.120 |
High resolution limit [Å] | 2.080 | 2.080 |
Rmerge | 0.077 | 0.380 |
Number of reflections | 13197 | 395 |
<I/σ(I)> | 18.7 | 2.03 |
Completeness [%] | 92.6 | 56.7 |
Redundancy | 4.5 | 2.3 |
CC(1/2) | 0.940 | 0.740 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 80% 2.7M sodium malonate, 20% 2.1M Malic acid |