5XXQ

Crystal structure of RBBP4: ZNF827 and its function in telomere

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	75.960, 59.780, 102.560
Unit cell angles	90.00, 95.07, 90.00

Refinement procedure

Resolution	40.700 - 1.900
R-factor	0.1745
Rwork	0.172
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3gfc
RMSD bond length	0.013
RMSD bond angle	1.233
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	102.158	40.700	2.000
High resolution limit [Å]	1.900	6.010	1.900
Rmerge		0.046	0.458
Rmeas	0.115	0.050	0.500
Rpim	0.045	0.019	0.197
Total number of observations	376009	16683	51922
Number of reflections	62445	2380	8490
<I/σ(I)>	9.7	21.5	3.3
Completeness [%]	86.2	98.9	80.8
Redundancy	6	7	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	283	0.2M Ammonium sulfate, 0.1M MES monohydrate pH 6.5 , 30%(w/v) Polyethylene glycol monomethyl ether 5,000