5XXP
Crystal structure of CbnR_DBD-DNA complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | AREA DETECTOR |
Collection date | 2016-11-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.9 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 186.457, 28.719, 73.153 |
Unit cell angles | 90.00, 111.65, 90.00 |
Refinement procedure
Resolution | 67.990 - 2.550 |
R-factor | 0.22164 |
Rwork | 0.219 |
R-free | 0.26327 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3k1m |
RMSD bond length | 0.008 |
RMSD bond angle | 1.366 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | BALBES |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 67.990 | 2.690 |
High resolution limit [Å] | 2.550 | 2.550 |
Number of reflections | 11461 | 3253 |
<I/σ(I)> | 13.52 | 3.27 |
Completeness [%] | 97.1 | 95.5 |
Redundancy | 3.3 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 30%(w/v) PEG4000, 0.1 M Tris-HCl pH 8.5, 0.2 M sodium acetate |