5XWR

Crystal Structure of RBBP4-peptide complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-29
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	73.840, 59.860, 101.529
Unit cell angles	90.00, 93.70, 90.00

Refinement procedure

Resolution	34.960 - 2.690
R-factor	0.198
Rwork	0.192
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xu7
RMSD bond length	0.009
RMSD bond angle	1.346
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.750
High resolution limit [Å]	2.689	2.690
Number of reflections	23165
<I/σ(I)>	6.1
Completeness [%]	93.5
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1M Bis-Tris, pH5.5, 25% PEG 3,350