5XWB

Crystal Structure of 5-Enolpyruvulshikimate-3-phosphate Synthase from a Psychrophilic Bacterium, Colwellia psychrerythraea

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-02-23
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 1 21 1
Unit cell lengths	61.798, 80.027, 82.271
Unit cell angles	90.00, 96.46, 90.00

Refinement procedure

Resolution	50.000 - 2.200
R-factor	0.14947
Rwork	0.146
R-free	0.20990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nvs
RMSD bond length	0.016
RMSD bond angle	1.764
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.200
Rmerge	0.089
Number of reflections	40348
<I/σ(I)>	25.7
Completeness [%]	99.9
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG 3350, soidum chloride, glycerol