5XW5

Crystal structure of budding yeast Cdc14p (C283S) bound to a Swi6p phosphopeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	CCD
Collection date	2009-12-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	82.930, 95.930, 138.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.595 - 1.850
R-factor	0.1918
Rwork	0.191
R-free	0.21660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xw4
RMSD bond length	0.006
RMSD bond angle	0.758
Data reduction software	iMOSFLM
Data scaling software	Aimless (0.5.32)
Phasing software	MOLREP
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.595	30.590	1.880
High resolution limit [Å]	1.850	10.130	1.850
Rmerge	0.059	0.019	0.442
Rmeas	0.068	0.022	0.533
Rpim	0.033	0.011	0.292
Number of reflections	91878
<I/σ(I)>	13.8
Completeness [%]	97.0	88.7	79.2
Redundancy	3.9	3.8	2.8
CC(1/2)	0.998	0.999	0.809

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	Bis-Tris, ammonium sulfate, PEG 4000