Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-13 |
| Detector | MAR CCD 130 mm |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 32 |
| Unit cell lengths | 69.452, 69.452, 129.670 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.100 - 2.170 |
| R-factor | 0.2245 |
| Rwork | 0.223 |
| R-free | 0.25670 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.078 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | Auto-Rickshaw |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.250 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Rpim | 0.281 | |
| Number of reflections | 37009 | 3682 |
| <I/σ(I)> | 46.8 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.6 | 11.1 |
| CC(1/2) | 0.861 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 289 | 6% PEG 8000, 12% ethylene glycol, 0.02 M glycine, 0.02 M DL-lysine, 0.02 M DL-serine, 0.02 M DL-alanine and 0.02 M sodium L-glutamate, 0.1 M MES/imidazole pH 6.9 |
