5XW2
Crystal structure of the Hydroxylase HmtN in C 1 2 1 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979228 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.130, 72.840, 53.750 |
| Unit cell angles | 90.00, 107.43, 90.00 |
Refinement procedure
| Resolution | 46.508 - 1.298 |
| R-factor | 0.1688 |
| Rwork | 0.168 |
| R-free | 0.18480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ggv |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.282 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.171 | |
| High resolution limit [Å] | 1.298 | 1.298 |
| Rmerge | 0.047 | 0.286 |
| Number of reflections | 111281 | |
| <I/σ(I)> | 18.79 | 3.88 |
| Completeness [%] | 97.4 | 94.8 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 10% (w/v) PEG 8000, 0.2M magnesium chloride, 0.1M Tris, pH 7.0 |
