5XVW

Crystal structure of AL2 PAL domain in complex with AtRing1a distal site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-13
Detector	RAYONIX MX-225
Wavelength(s)	0.97853
Spacegroup name	P 1
Unit cell lengths	43.569, 52.618, 72.138
Unit cell angles	102.66, 97.82, 109.04

Refinement procedure

Resolution	27.508 - 1.849
R-factor	0.1765
Rwork	0.175
R-free	0.20350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.948
Data reduction software	HKL-2000
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.849
Number of reflections	47282
<I/σ(I)>	25.6
Completeness [%]	96.2
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	299	15% PEG 4000, 0.1 M sodium citrate, pH 5.0,