5XVP
E. fae Cas1-Cas2/prespacer/target ternary complex revealing the fully integrated states
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-03-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97910 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 64.870, 212.294, 512.199 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.030 - 3.000 |
R-factor | 0.19557 |
Rwork | 0.194 |
R-free | 0.23264 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.580 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.000 |
Number of reflections | 68246 |
<I/σ(I)> | 8.6 |
Completeness [%] | 99.1 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.5 | 100 mM sodium acetate, 100 mM sodium citrate, pH 4.8, and 4-7% (w/v) PEG 4000 |