5XVO
E. fae Cas1-Cas2/prespacer/target ternary complex revealing DNA sampling and half-integration states
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.91910 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 131.893, 124.800, 157.905 |
Unit cell angles | 90.00, 106.50, 90.00 |
Refinement procedure
Resolution | 48.950 - 3.100 |
R-factor | 0.19587 |
Rwork | 0.194 |
R-free | 0.23334 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.576 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | SHELXCD |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.100 |
Number of reflections | 82547 |
<I/σ(I)> | 13.2 |
Completeness [%] | 97.3 |
Redundancy | 14.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.5 | 100 mM sodium acetate, 100 mM sodium citrate, pH 6.2, and 4-7% (w/v) PEG 4000 |