5XVI
Crystal Structure of Aspergillus niger Apo- Glutamate Dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-08-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 92.800, 92.820, 111.690 |
| Unit cell angles | 103.46, 94.17, 120.13 |
Refinement procedure
| Resolution | 35.000 - 2.800 |
| R-factor | 0.22987 |
| Rwork | 0.226 |
| R-free | 0.31011 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3sbo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.429 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.060 | 0.280 |
| Number of reflections | 69072 | 6502 |
| <I/σ(I)> | 11.2 | 3.6 |
| Completeness [%] | 92.0 | 86 |
| Redundancy | 2 | 1.6 |
| CC(1/2) | 0.990 | 0.840 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 20% PEG 3350, 0.1 M NaCl, 0.1 M Tris-Cl, pH 8.5, 0.01 M BaCl2 |
