5XVH

Crystal structure of the NADP+ and tartrate-bound complex of L-serine 3-dehydrogenase from the hyperthermophilic archaeon Pyrobaculum calidifontis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-06
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	C 1 2 1
Unit cell lengths	120.965, 57.509, 56.412
Unit cell angles	90.00, 106.91, 90.00

Refinement procedure

Resolution	28.700 - 1.570
R-factor	0.173
Rwork	0.173
R-free	0.17900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3w6u
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.600
High resolution limit [Å]	1.570	1.570
Rmerge	0.064	0.269
Number of reflections	49918
<I/σ(I)>	15.5
Completeness [%]	96.5
Redundancy	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	2.0M ammonium sulfate, 100mM acetate buffer pH 4.6