5XV5
Crystal structure of Rib7 mutant S88E from Methanosarcina mazei
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2010-05-13 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 |
Unit cell lengths | 116.160, 116.160, 380.801 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.500 |
R-factor | 0.24795 |
Rwork | 0.246 |
R-free | 0.28116 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xux |
RMSD bond length | 0.013 |
RMSD bond angle | 1.656 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.478 | |
Number of reflections | 65992 | |
<I/σ(I)> | 18.2 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 2.8 | 5.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20-25 % Tacsimate (pH 7.0), 2 % PEG 3350, and 100 mM HEPES (pH 7.0) |