5XV2
Crystal structure of Rib7 mutant D33A from Methanosarcina mazei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2008-08-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 70.147, 70.147, 217.458 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.907 - 2.000 |
| R-factor | 0.23579 |
| Rwork | 0.235 |
| R-free | 0.25172 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xux |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.585 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.744 | |
| Number of reflections | 22298 | 43100 |
| <I/σ(I)> | 65.9 | 4.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 20.7 | 20 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20-25 % Tacsimate (pH 7.0), 2 % PEG 3350, and 100 mM HEPES (pH 7.0) |
