5XV1
Crystal structure of ATG101-ATG13HORMA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.483, 125.505, 101.038 |
| Unit cell angles | 90.00, 101.66, 90.00 |
Refinement procedure
| Resolution | 32.984 - 2.508 |
| R-factor | 0.2128 |
| Rwork | 0.210 |
| R-free | 0.25120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xyu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.926 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.508 |
| Rmerge | 0.055 |
| Rmeas | 0.062 |
| Rpim | 0.037 |
| Total number of observations | 229055 |
| Number of reflections | 36707 |
| <I/σ(I)> | 6 |
| Completeness [%] | 97.8 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M HEPES (pH7.5), 0.2M MgCl2, 6-8% (w/v) PEG 3350 |
