5XUW
Crystal structure of lysozyme from Equus asinus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17B1 |
| Synchrotron site | SSRF |
| Beamline | BL17B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97775 |
| Spacegroup name | P 32 |
| Unit cell lengths | 57.543, 57.543, 73.359 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.830 - 1.760 |
| R-factor | 0.19407 |
| Rwork | 0.193 |
| R-free | 0.22083 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1el1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.303 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Number of reflections | 26974 | |
| <I/σ(I)> | 19.562 | |
| Completeness [%] | 99.9 | |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 25% PEG 3350, 0.2 M NaCl, 0.1 M Bis-tris |
