5XU7
Crystal structure of Escherichia coli holo-[acyl-carrier-protein] synthase (AcpS)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 97.620, 97.620, 96.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.840 |
| R-factor | 0.17719 |
| Rwork | 0.175 |
| R-free | 0.21357 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qmn |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.990 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.810 | 1.940 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.083 | 0.603 |
| Rpim | 0.024 | 0.176 |
| Number of reflections | 40765 | 5866 |
| <I/σ(I)> | 19 | 4.4 |
| Completeness [%] | 99.4 | 99.5 |
| Redundancy | 12.7 | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8 | 277 | 10mM Tris-HCl pH 8.0, 100mM NaCl, 10mM MgCl2, 1mM DTT |
