5XU1
Structure of a non-canonical ABC transporter from Streptococcus pneumoniae R6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97775 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 141.053, 154.987, 205.892 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.300 |
| R-factor | 0.23643 |
| Rwork | 0.234 |
| R-free | 0.28922 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f3o |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.298 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 34560 | |
| <I/σ(I)> | 13.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 285 | 0.2 M ammonium sulfate, 0.1 M sodium acetate, 25-31% v/v PEG 550 MME, 0.01 M Cadmium chloride |
