5XTX
Crystal Structure of Transketolase in complex with TPP intermediate VII from Pichia Stipitis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-24 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.6500 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 101.201, 185.345, 98.957 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.902 - 1.049 |
| R-factor | 0.1305 |
| Rwork | 0.130 |
| R-free | 0.13900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hyv |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.453 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.049 |
| Number of reflections | 425007 |
| <I/σ(I)> | 35.3 |
| Completeness [%] | 99.4 |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M MES, 0.1M NaCl, PEG 400 |
