5XTQ
Crystal structure of baculoviral sulfhydryl oxidase P33 (H227D mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.098, 76.040, 80.631 |
| Unit cell angles | 84.61, 71.27, 69.00 |
Refinement procedure
| Resolution | 27.570 - 2.040 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qzy |
| Data reduction software | HKL-2000 (V0.97.644) |
| Data scaling software | HKL-2000 (V0.97.644) |
| Phasing software | PHENIX (1.11.1_2575) |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.035 | 0.102 |
| Total number of observations | 143698 | |
| Number of reflections | 75844 | |
| <I/σ(I)> | 18.9 | |
| Completeness [%] | 96.5 | 95.8 |
| Redundancy | 1.9 | 1.9 |
| CC(1/2) | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.4 | 293 | PEG3350 |
