5XTO
Crystal structure of baculoviral sulfhydryl oxidase P33 (H114A mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.132, 152.642, 75.885 |
| Unit cell angles | 90.00, 110.92, 90.00 |
Refinement procedure
| Resolution | 37.550 - 2.560 |
| R-factor | 0.21 |
| Rwork | 0.209 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qzy |
| Data reduction software | HKL-2000 (V0.97.644) |
| Data scaling software | HKL-2000 (V0.97.644) |
| Phasing software | PHENIX (1.11.1_2575) |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 2.650 |
| High resolution limit [Å] | 2.555 | 2.560 |
| Rmerge | 0.079 | 0.484 |
| Total number of observations | 142090 | |
| Number of reflections | 37851 | |
| <I/σ(I)> | 6.4 | |
| Completeness [%] | 93.2 | 91.9 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 293 | PEG3350 |
