5XTA
Crystal structure of lpg1832, a VirK family protein from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.200, 74.840, 85.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.22025 |
| Rwork | 0.218 |
| R-free | 0.26010 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.734 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 85.000 |
| High resolution limit [Å] | 2.000 |
| Number of reflections | 30567 |
| <I/σ(I)> | 10.7 |
| Completeness [%] | 93.3 |
| Redundancy | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277 | 0.2 M Lithium sulfate monohydrate, 0.1 M Bis-Tris pH 5.5, 25% w Polyethylene glycol 3,350 |
