5XSX
Crystal structure of an archaeal chitinase in the substrate-complex form (P212121)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2014-11-15 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.932, 145.196, 152.733 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.626 - 2.642 |
R-factor | 0.2315 |
Rwork | 0.230 |
R-free | 0.25910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xsw |
RMSD bond length | 0.002 |
RMSD bond angle | 0.544 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.710 |
High resolution limit [Å] | 2.650 | 2.650 |
Number of reflections | 50103 | |
<I/σ(I)> | 17.4 | |
Completeness [%] | 100.0 | |
Redundancy | 13.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | Tris-HCl (pH 8.5), NH4H2PO4 |