5XSV
Crystal structure of an archaeal chitinase in the ligand-free form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2014-02-14 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 126.925, 104.730, 156.463 |
| Unit cell angles | 90.00, 113.43, 90.00 |
Refinement procedure
| Resolution | 41.803 - 3.723 |
| R-factor | 0.2793 |
| Rwork | 0.278 |
| R-free | 0.29830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xsw |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.570 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.830 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Number of reflections | 39375 | |
| <I/σ(I)> | 4.8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | MES monohydrate (pH 6.5), CoCl2, (NH4)2SO4 |
