5XSQ

Crystal Structure of the Marburg Virus Nucleoprotein Core Domain Chaperoned by a VP35 Peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97922
Spacegroup name	P 32
Unit cell lengths	101.320, 101.320, 96.330
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.907 - 2.600
R-factor	0.2156
Rwork	0.214
R-free	0.24420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ypi
RMSD bond length	0.005
RMSD bond angle	0.990
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.141	2.307
Rpim	0.061	0.993
Number of reflections	35200
<I/σ(I)>	14.937	1.209
Completeness [%]	100.0	100
Redundancy	6.3	6.3
CC(1/2)	0.990	0.457

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M sodium citrate tribasic monohydrate, pH 8.3, 20% (w/v) PEG 3350