5XSL
Crystal structure of the PWWP domain of human hepatoma-derived growth factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2015-12-09 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 79.490, 79.490, 105.099 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.300 |
| R-factor | 0.22854 |
| Rwork | 0.225 |
| R-free | 0.28218 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qby |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.853 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.081 | 0.438 |
| Number of reflections | 3320 | |
| <I/σ(I)> | 21.89 | 3.59 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.8 | 11.9 |
| CC(1/2) | 0.958 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 281 | ammonium sulfate, Tris, PEG 3350 |
