5XS9
Crystal structure of Mycobacterium smegmatis BioQ
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NFPSS BEAMLINE BL18U |
Synchrotron site | NFPSS |
Beamline | BL18U |
Temperature [K] | 195 |
Detector technology | PIXEL |
Collection date | 2017-03-31 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9778 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 61.041, 190.179, 83.413 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
R-factor | 0.1816 |
Rwork | 0.179 |
R-free | 0.22908 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.796 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.900 |
Number of reflections | 38389 |
<I/σ(I)> | 41.2 |
Completeness [%] | 99.7 |
Redundancy | 12.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.15 M DL-malic acid (pH 7.0), 0.1 M imidazole (pH 7.0), 22% (v/v) PEG mono-methyl ether 550 |