5XS6
Crystal Structure of Transketolase in complex with TPP Pichia Stipitis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-24 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.7 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 101.062, 184.795, 99.088 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.556 - 0.972 |
R-factor | 0.1586 |
Rwork | 0.158 |
R-free | 0.16630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hyv |
RMSD bond length | 0.006 |
RMSD bond angle | 1.020 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 0.890 |
High resolution limit [Å] | 0.870 | 0.870 |
Rmerge | 0.054 | 0.969 |
Number of reflections | 712546 | |
<I/σ(I)> | 30.6 | 1.7 |
Completeness [%] | 95.7 | 93.8 |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M MES, 0.1M NaCl, PEG 400 |