5XS0
Structure of a ssDNA bound to the outer DNA binding site of RAD52
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 100.224, 164.408, 166.087 |
Unit cell angles | 90.00, 90.19, 90.00 |
Refinement procedure
Resolution | 48.529 - 3.000 |
R-factor | 0.2225 |
Rwork | 0.221 |
R-free | 0.25060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kn0 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.571 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.630 | 3.050 |
High resolution limit [Å] | 3.000 | 3.000 |
Number of reflections | 107806 | 5281 |
<I/σ(I)> | 11.8 | 1.8 |
Completeness [%] | 99.9 | 98.7 |
Redundancy | 3.8 | 3.8 |
CC(1/2) | 0.997 | 0.733 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 285 | lithium citrate, PEG 3,350 |