5XRS

Crystal structure of A/Minnesota/11/2010 (H3N2) influenza virus hemagglutinin in complex with LSTc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.033
Spacegroup name	I 21 3
Unit cell lengths	292.980, 292.980, 292.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.324 - 2.907
R-factor	0.1892
Rwork	0.187
R-free	0.22520
RMSD bond length	0.005
RMSD bond angle	0.934
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.950
High resolution limit [Å]	2.900	2.900
Rmerge	0.164	0.899
Rpim	0.050	0.787
Number of reflections	91393	4740
<I/σ(I)>	23.6	1.3
Completeness [%]	100.0	100
Redundancy	19.5
CC(1/2)	0.563	0.647

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M calcium acetate, 18% (w/v) polyethylene glycol PEG 8000, 0.1 M sodium cacodylate (pH 6.5)