5XRQ
Crystal structure of human monoclonal antibody H3v-47
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-03-20 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.033 |
Spacegroup name | P 31 |
Unit cell lengths | 135.126, 135.126, 78.530 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.920 - 2.600 |
R-factor | 0.1825 |
Rwork | 0.180 |
R-free | 0.21440 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.170 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.610 |
High resolution limit [Å] | 2.570 | 2.570 |
Rmerge | 0.093 | 0.412 |
Rpim | 0.058 | 0.256 |
Number of reflections | 50148 | 2143 |
<I/σ(I)> | 8.1 | 1 |
Completeness [%] | 98.2 | 83.4 |
Redundancy | 3.4 | |
CC(1/2) | 0.891 | 0.891 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 20% (w/v) polyethylene glycol (PEG) 6000, 0.1 M sodium citrate (pH 5.0) |