5XR9
Crystal structure of Human Hsp90 with FS6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-21 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 67.225, 90.524, 98.025 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.612 - 1.501 |
R-factor | 0.1842 |
Rwork | 0.184 |
R-free | 0.19530 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.057 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.6.2_432) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 33.612 |
High resolution limit [Å] | 1.501 |
Number of reflections | 48036 |
<I/σ(I)> | 10.7 |
Completeness [%] | 99.9 |
Redundancy | 14.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 20-25%(w/v) PEG 2000 monomethyl ether, 200mM magnesium chloride, 100mM sodium cacodylate, pH 6.5 |