5XR7

Crystal structure of RabA1a (Q72K) in complex with GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-26
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	37.570, 43.046, 67.609
Unit cell angles	86.66, 74.07, 82.28

Refinement procedure

Resolution	29.415 - 2.600
R-factor	0.2229
Rwork	0.216
R-free	0.28550
RMSD bond length	0.010
RMSD bond angle	1.116
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600
Number of reflections	12784
<I/σ(I)>	26.9
Completeness [%]	97.7
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1 M MES (pH5.5), 10 mM magnesium acetate, 2.2 M ammonium sulfate