5XR6

Crystal structure of RabA1a in complex with GppNHp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-26
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	37.634, 48.416, 68.508
Unit cell angles	88.53, 74.15, 84.37

Refinement procedure

Resolution	27.183 - 2.600
R-factor	0.2092
Rwork	0.203
R-free	0.26530
RMSD bond length	0.008
RMSD bond angle	1.127
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600
Number of reflections	14606
<I/σ(I)>	28.6
Completeness [%]	96.5
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	0.1 M MES (pH5.5), 10 mM magnesium acetate, 2.2 M ammonium sulfate