5XQV
Crystal structure of Notched-fin eelpout type III antifreeze protein A20L mutant (NFE6, AFP), P21 form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-12-23 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.905 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 28.475, 33.735, 34.391 |
Unit cell angles | 90.00, 112.04, 90.00 |
Refinement procedure
Resolution | 31.880 - 0.970 |
R-factor | 0.13 |
Rwork | 0.129 |
R-free | 0.15800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xqn |
RMSD bond length | 0.007 |
RMSD bond angle | 1.305 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.000 |
High resolution limit [Å] | 0.970 | 0.970 |
Rmerge | 0.068 | 0.317 |
Number of reflections | 34361 | |
<I/σ(I)> | 28.4 | 3.1 |
Completeness [%] | 95.5 | 86 |
Redundancy | 2.7 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 2.3 M Ammonium sulfate, 0.1 M Na-Citrate pH5.0 |