5XQO

Crystal structure of a PL 26 exo-rhamnogalacturonan lyase from Penicillium chrysogenum complexed with tetrameric substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-26
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.0
Spacegroup name	P 42 21 2
Unit cell lengths	166.437, 166.437, 171.932
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	119.580 - 3.200
R-factor	0.19469
Rwork	0.191
R-free	0.26432
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xq3
RMSD bond length	0.011
RMSD bond angle	1.616
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	119.580	3.260
High resolution limit [Å]	3.200	3.200
Rmerge	0.092	0.425
Number of reflections	40427	1960
<I/σ(I)>	31.4	4.83
Completeness [%]	99.9	100
Redundancy	7.2	7.3
CC(1/2)		0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	3.1M Sodium acetate