5XQE

Crystal structure of Human Hsp90 with FS3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	I 2 2 2
Unit cell lengths	67.772, 90.539, 98.032
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.886 - 1.699
R-factor	0.1865
Rwork	0.186
R-free	0.20000
RMSD bond length	0.006
RMSD bond angle	1.022
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.6.2_432)

Data quality characteristics

	Overall
Low resolution limit [Å]	33.886
High resolution limit [Å]	1.699
Number of reflections	33567
<I/σ(I)>	3.9
Completeness [%]	99.9
Redundancy	14.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	25%(w/v) PEG 2000 monomethyl ether, 200mM magnesium chloride, 100mM sodium cacodylate