5XQ3

Crystal structure of a PL 26 exo-rhamnogalacturonan lyase from Penicillium chrysogenum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-20
Detector	RAYONIX MX-225
Wavelength(s)	0.979
Spacegroup name	P 42 21 2
Unit cell lengths	167.471, 167.471, 172.289
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	120.090 - 2.850
R-factor	0.17329
Rwork	0.171
R-free	0.23656
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.637
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0151)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	120.090	2.900
High resolution limit [Å]	2.850	2.850
Rmerge	0.117
Number of reflections	57725	2860
<I/σ(I)>	33	3
Completeness [%]	100.0	100
Redundancy	20	15
CC(1/2)		0.886

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	2.9 M Sodium Acetate