5XPZ

Structural basis of kindlin-mediated integrin recognition and activation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 32 2 1
Unit cell lengths	75.427, 75.427, 383.871
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.652 - 2.601
R-factor	0.2427
Rwork	0.241
R-free	0.28330
Structure solution method	MAD
RMSD bond length	0.002
RMSD bond angle	0.559
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.077	0.046	0.854
Rmeas	0.084	0.050	0.920
Rpim	0.033	0.020	0.333
Number of reflections	37581
<I/σ(I)>	12.7
Completeness [%]	93.0	93.3	94.2
Redundancy	6.2	5.8	6.8
CC(1/2)		0.997	0.639

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	0.2 M potassium chloride, 0.05 M HEPES pH 7.5, 35% v/v pentaerythritol propoxylate